RESEARCHES
I study plant proteins which inhibit hydrolytic enzymes of various
organisms:
Inhibitors are of interest as:
The inhibitors are well studied in various
plants, but, generally, molecular structures of single
inhibitors are studied much more then their natural variability
in plant taxa.
The simple and sensitive methods for detection of inhibitors
of
alpha-amylases, serine
and cysteine proteinases and lectins
among plant proteins separated by electrophoresis, isoelectric
focusing or thin layer gel-filtration were elaborated (Konarev,
1985,
1986; Konarev et al.,
1991,
2000,
2002).
These methods were used in study of the polymorphism and
natural variability of main inhibitor systems in wild and cultivated species
of
wheat and related cereals, rice, potato,
Vigna and Phaseolus,
sunflower, various
Compositae,
other Asteridae,
Gymnospermae and for
identification of novel inhibitor types. Small (1.5 kDa)
cyclic trypsin inhibitor
from sunflower seeds can be one of the examples of such inhibitors.
Besides, I like to travel.
I collect wild plants or simply vizit exotic places...
EXAMPLES OF RESEARCHES
|
Variability of trypsin inhibitors in Vigna
and Phaseolus species.
Isoelectric focusing of seed proteins followed by detection of
inhibitors with use of
gelatin replicas method. |
|
The polymorphism of chymotrypsin/subtilisin inhibitors in several bread
wheat varieties. Endosperm proteins were extracted with water and
separated by isoelectric focusing in PAG. Protein bands were transferred
from gel to the gelatine replica by diffusion and the latter was
developed by chymotrypsin. |
 |
Presentation of methods for detection of insect alpha-amylase and
proteinase inhibitors among Vigna proteins in Laboratory
of Plant Evolutionary Dynamics in NIAR,
Head Dr. Duncan.A.Vaughan (right), Tsukuba, Japan.
|
KEY WORDS
insect amylase inhibitors, proteinase inhibitors,
trypsin inhibitors, chymotrypsin inhibitors, subtilisin
inhibitors, cysteine proteinase inhibitors, papain inhibitors,
ficin inhibitors, lectins, methods for detection of inhibitors,
plant diversity, plant evolution, plant
systematics, plant resistance, cereals, legumes, potato, sunflower,
Compositae, Asteridae, Gymnospermae, Triticum, Aegilops, Secale, Hordeum,
Vigna, Phaseolus, Oryzae, Helianthus, Carthamus, Cosmos,
Zinnia, Centaurea, Veronica,
Anthriscus
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Используются технологии
uCoz
