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CURRICULUM VITAE
SPECIALIZATION
MAILING ADDRESS
Dr. Al.Konarev,
All-Russian Institute of Plant Protection (VIZR)
shosse Podbelskogo, 3, St.Petersburg 196609, RUSSIA
e-mail: al_konarev@hotmail.com
home-page: http://alvaskon.chat.ru
PERSONAL
Birth:
January 15, 1956, RUSSIA
Nationality: Russian
Marriage: Married
Children: Son
Languages: Russian, English
EDUCATION
19.11.1992
Doctor of Sciences degree in biology, Scientific Council at the
Russian Academy of Sciences
A.N.Bach Institute of Biochemistry (Moscow).
Thesis: "Systems of hydrolase inhibitors in
cereals: organisation, functions and evolutionary variability."
17.03.1983
PhD degree in biology, Scientific Council at the Leningrad (St. Petersburg)
State University.
Thesis: "The nature of alpha-amylase inhibitors in
relation to evolution and immunity of wheat and other cereals"
1979-1982
Postgraduate Courses at the All-Russian Institute for Plant Protection and
at N.I.Vavilov Institute of Plant Industry,
Leningrad, USSR. Majoring in plant protein biochemistry, enzymology, systematics,
genome analysis, plant immunity
1973-1978
MSc degree in plant physiology and biochemistry, Leningrad State University,
Faculty of Biology.
Thesis: "Insect and human alpha-amylase inhibitors
in wheat."
PRESENT POSITION
Leading Research Scientist,
All-Russian Institute of Plant Protection,
Department of Entomology and Plant Immunity to Pests,
shosse Podbelskogo, 3, Pushkin, St.Petersburg 196609, RUSSIA
FAX: 7-812-470-51-10
Experimental base: N.I.Vavilov Institute
of Plant Industry,Department of Molecular Biology, Bol. Morskaya str.
44, St.Petersburg 190000 RUSSIA
EMPLOYMENT RECORD
1982-2002
Research/Senior/Leading Research Scientist, All-Russian
Institute for Plant Protection,
Department of Entomology and Plant Immunity to
Pests. Shosse; Podbelskogo, 3,Pushkin, St.Petersburg, 196609, RUSSIA
PRESENT AND CURRENT RESEARCH AREAS,
SCHOLARLY ACTIVITIES,
PROJECTS AND GRANTS
2002
IACR-Long-Ashton Research Station, University of Bristol, UK
Royal Society grant for 6 months (Photo)
Study of proteinase inhibitors in various Asteridae and Gymnospermae at
Crop Performance and Improvement Division (Head Prof. P.R.Shewry)
Results will be published in Phytochemistry (2004a & b, in press)
2000
IACR-Long-Ashton Research Station, University of Bristol, UK
Royal Society grant for 6 months
Study of proteinase inhibitors in sunflower, safflower
and other Compositae plants at
Seed Structure and Composition Lab (Head Prof. P.R.Shewry)
Results were published in Phytochemistry 59 (2002)
1997-1998
JIRCAS (Japan International Research Center of Agricultural
Sciences) fellowship.
National Institute of Agrobiological Resources,
Plant Evolutionary Dynamics Lab., (Head Dr.Duncan Vaughan)
Tsukuba, Japan
(6 months Sept 1997-March 1998).
Responsible: study of proteinase and
amylase inhibitors in Vigna, Phaseolus and Oryzae species,
detection of inhibitors, analysis of
their variability and interaction
with mammal, microbial and insect enzymes in relation with
plant evolution and resistance to harmful organisms.
Results were reported on
EUCARPIA Congress(Viterbo, Italy, 1998a) and published in
EUPHYTICA, 2002
1995-1997
Grant from Royal Society, Long-Ashton Research Station,
(Photo) Bristol, UK (6 months total).
Participant of Joint Project "SUNFLOWER PROTEINS".
Responsible: study of proteinase inhibitors from sunflower:
detection, purification, analysis of variability in cultivated and wild
species,genetic analysis, interaction with proteinases
from pathogenic fungi. Discovering of
low-molecular (1.5 kDa) TI in sunflower.
Study of genetic control and biochemical
properties of sunflower albumins.
Results were reported at EUCARPIA
Congress (Viterbo, Italy, 1998b) and published in
J Phytopathol (1999), J.Mol.Biol. (1999), Theor Appl Genet (2000) and Euphytica (2003)
1993-1994
Grant from Russian Fund for Fundamental Researches, All-Russian
Institute for Plant Protection; responsible:
analysis of heterogeneity,
variability and chromosome control of insect and plant
cysteine proteinase inhibitors in wheat, rye, barley and
other cereals.
1986-1992
Proteinase inhibitors in vegetative
organs of cereals; project leader; responsible:
analysis of component composition
and genetic control of trypsin inhibitors (TIs)
from vegetative organs of wheat and related cereals.
1982-1992
TIs in endosperm of wheat and
related cereals; project leader; responsible:
identification of TIs among endosperm proteins in
di-, tetra- and hexaploid wheats, purification and
studying of inhibitors from different wheat
species.
Analysis of inhibitors in species
and varieties of Triticum L., Aegilops L., Secale L. and
other cereals; analysis of
genome and chromosome
control and inheritance of TIs;
study of defence role of TIs
against insect pests.
1989-1991
Lectins and proteinase inhibitors
in cereals; project leader; responsible:
development of simple and sensitive
methods of simultaneous detection of
lectins and proteinase inhibitors
in PAGE and IEF plant protein patterns
(USSR patent). Research of lectins and comparison of
lectins and inhibitors in wheat and other cereals.
1988-1990
Heterogeneity of hydrolases in
relation to inhibitors: project leader; responsible: elaboration
of "cross" methods of analysis of interaction of components of complex
patterns of enzymes with inhibitor components (USSR patent); studying of interaction of components of insect digestive and endogenous wheat alpha-amylases and proteinases with components of wheat inhibitors.
1984-1989
Inhibitors of chymotrypsin and
subtilisin in cereals; project leader;
responsible: analysis of heterogeneity, chromosome control, character of inheritance and
evolutionary variability of inhibitors of chymotrypsin and subtilisin in Triticum L., Aegilops L. and Secale L. species;
purification and studying of biochemical properties of inhibitors; elaboration of wheat variety identification method
by IEF-patterns of chymotrypsin/subtilisin
inhibitors (USSR patent). Results were presented at the III International Symp. Biochemical Identification of Varieties (USSR, 1987)
1982-1990
Digestive proteinases of insects and
their inhibitors in cereals; project leader; responsible: analysis
of digestive proteinases of insects damaging wheat grain;
revealing and studying of wheat grain inhibitors of
insect proteinases; discovering the trypsin- and chymotrypsin-like and
cysteine digestive proteinases in wheat pests. Results were presented at the I International Symp. on Sunn pest (Turkey,
1988)
1982-1984
Inhibitors of insect and plant cysteine proteinases
in wheat and related cereals; project
leader; responsible: discovering of inhibitors of cysteine proteinases
of insects and endogenous cysteine proteinase of
germinating wheat grain and papain in wheats;
analysis of evolutionary variability and chromosome
control of cysteine proteinase inhibitors in wheat.
1982-1989
Methods
for identification of proteinase inhibitors; project
leader; responsible: elaboration of complex
of high sensitive, simple and universal methods
for identification of serine and cysteine
proteinase inhibitors after electrophoresis, isoelectric
focusing or thin layer gel-filtration of plant
or insect proteins (USSR patents)
1979-1982
Alpha-amylase
inhibitors in wheats and other cereals; project leader;
responsible: elaboration of simple and high sensitive methods for detection of alpha-amylase
inhibitors in protein patterns after electrophoresis, isoelectric focusing or thin layer gel-filtration (USSR patents);
analysis of insect and mammal alpha-amylase inhibitors in wheat
and related cereals (heterogeneity, evolutionary
variability, chromosome control, inheritance); design of scheme of evolution of insect alpha-amylase inhibitor system in
genus Triticum L.; discovering of endogenous wheat alpha-amylase
inhibitors in wheats and other Triticeae Dum.
cereals (1981), analysis of properties, distribution and variability
of these inhibitors (1982).
INTERNATIONAL CONFERENCES
XI-th Int.CONGRESS ON PLANT-MICROBE INTERACTIONS (St.Petersburg,
Russia, July 18-26, 2003).
Presentation: Distribution and polymorphism of fungal serine
proteinase inhibitors in the Asteridae.
GLUTEN 2000, International Workshop (University of Bristol,
April 2-7, 2000 ).
Presentation: Insect and fungal enzyme inhibitors
in study of variability, evolution and resistance of wheat and other Triticeae Dum. cereals
THE MENDEL CENTENARY CONGRESS 2000 (100 YEARS OF GENETICS
FOR PLANT BREEDING - MENDEL, MEIOSIS AND MARKER)
(Brno, Czech Republic, March 7-10, 2000).
Presentation: Insect and fungal enzyme inhibitors in
study of plant variability and evolution.
Oral presentations:
XV EUCARPIA
Congress ( Viterbo, Italy, 20-25 Sept.1998).
GENETICS AND BREEDING FOR CROP QUALITY
AND RESISTANCE.
1. Variability of the inhibitors of serine, cysteine
proteinases and insect
alpha- amylases in Vigna and Phaseolus.
2. Polymorphism of Inhibitors of
Hydrolytic Enzymes Present in Cereal and
Sunflower
Seeds
XIV EUCARPIA Congress
ADAPTATION IN PLANT BREEDING
(Juvaskyla, Finland, July 31-August 4., 1995).
Interaction of insect digestive
enzymes with
protein inhibitors from plants and host-parasite
coevolution
I INTERNATIONAL SYMPOSIUM ON SUNN PEST
(Turkey, Tekirdag, June 1988). Inhibitors of Sunn pest
(Eurygaster integriceps Put.) and other insect amylases and proteinases
in wheat and related cereals
REFEREES
Prof. P.R.Shewry
Associate Director of Rothamsted Research
Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ , UK
E-mail: peter.shewry@bbsrc.ac.uk Fax:
+44 (0) 1582 760981
DSci M.A.Belozersky
Moscow State University, Institute of Physical-Chemical
Biology, Moscow, 117234, Russia
E-mail: mbeloz@genebee.msu.su
Fax: 095-939-31-81
Dr. D.A.Vaughan
Head of Crop Evolutionary Dynamics Team National Institute of
Agrobiological Sciences (NIAS)
Kannondai 2-1-2 Tsukuba, Ibaraki 305-8602 Japan
E-mail: duncan@affrc.go.jp
Fax: 81-298-38-7408
http://delphi.phys.univ-tours.fr/Prolysis/ - Protease inhibitors
http://www.biochemist.com/home.htm
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